4n9n

X-ray diffraction
2.9Å resolution

Crystal Structure of Saccharomyces cerevisiae Upc2 Transcription Factor fused with T4 Lysozyme

Released:
Entry authors: Yang H, Im YJ

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Sterol uptake control protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 438 amino acids
Theoretical weight: 49.86 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q12151 (Residues: 598-714, 726-878; Coverage: 30%)
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
Gene names: E, MOX4, UPC2, YD8142.14, YD8142B.05, YDR213W
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P32
Unit cell:
a: 67.17Å b: 67.17Å c: 257.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.255 0.251 0.272
Expression system: Escherichia coli