4n90

X-ray diffraction
3.3Å resolution

Crystal structure of ternary complex of TRAIL, DR5, and Fab fragment from a DR5 agonist antibody

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tumor necrosis factor receptor superfamily member 10B Chains: R, S, T
Molecule details ›
Chains: R, S, T
Length: 126 amino acids
Theoretical weight: 14.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14763 (Residues: 57-182; Coverage: 33%)
Gene names: DR5, KILLER, TNFRSF10B, TRAILR2, TRICK2, UNQ160/PRO186, ZTNFR9
Sequence domains: TNFR/NGFR cysteine-rich region
Structure domains: Tumor Necrosis Factor Receptor, subunit A, domain 2
Tumor necrosis factor ligand superfamily member 10 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 168 amino acids
Theoretical weight: 19.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P50591 (Residues: 114-281; Coverage: 60%)
Gene names: APO2L, TNFSF10, TRAIL
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
Fab light chain Chains: E, G, I
Molecule details ›
Chains: E, G, I
Length: 215 amino acids
Theoretical weight: 23.41 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins
Fab heavy chain Chains: D, F, H
Molecule details ›
Chains: D, F, H
Length: 224 amino acids
Theoretical weight: 23.77 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P6122
Unit cell:
a: 152.006Å b: 152.006Å c: 613.155Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.229 0.286
Expression systems:
  • Escherichia coli
  • Not provided