PDBe 4n7y

X-ray diffraction
2.16Å resolution

Crystal structure of 14-3-3zeta in complex with a 8-carbon-linker cyclic peptide derived from ExoS

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 26.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63104 (Residues: 1-230; Coverage: 94%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Exoenzyme S Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 1.19 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided
UniProt:
  • Canonical: Q93SQ3 (Residues: 420-430; Coverage: 2%)
Gene name: exoS

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 72.51Å b: 103.52Å c: 114.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.256
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided