4n6f

X-ray diffraction
2.25Å resolution

Crystal structure of Amycolatopsis orientalis BexX complexed with G6P

Released:

Function and Biology Details

Reaction catalysed:
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H(2)O
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112871 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
thiazole synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 27.13 KDa
Source organism: Amycolatopsis orientalis subsp. vinearia
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D7RFL7 (Residues: 1-256; Coverage: 100%)
Gene name: bexX
Sequence domains: Thiazole biosynthesis protein ThiG
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P43212
Unit cell:
a: 168.94Å b: 168.94Å c: 42.367Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.222
Expression system: Escherichia coli BL21(DE3)