4n43

X-ray diffraction
3.8Å resolution

Human enterovirus 71 uncoating intermediate captured at atomic resolution

Released:
Source organism: Enterovirus A71

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assemblies composition:
hetero 180-mer (preferred)
hetero trimer
hetero pentadecamer
hetero octadecamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Capsid protein VP1 Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 32.7 KDa
Source organism: Enterovirus A71
UniProt:
  • Canonical: M9XM90 (Residues: 566-862; Coverage: 14%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP2 Chain: B
Molecule details ›
Chain: B
Length: 254 amino acids
Theoretical weight: 27.73 KDa
Source organism: Enterovirus A71
UniProt:
  • Canonical: Q9WQJ0 (Residues: 70-323; Coverage: 12%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP3 Chain: C
Molecule details ›
Chain: C
Length: 242 amino acids
Theoretical weight: 26.44 KDa
Source organism: Enterovirus A71
UniProt:
  • Canonical: Q9WPJ0 (Residues: 324-565; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P4232
Unit cell:
a: 352.416Å b: 352.416Å c: 352.416Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.245 0.274