X-ray diffraction
1.97Å resolution

Crystal structure of D-isomer specific 2-hydroxyacid dehydrogenase family protein from Klebsiella pneumoniae 342

Source organism: Klebsiella pneumoniae 342
Entry authors: Bacal P, Shabalin IG, Cooper DR, Majorek KA, Osinski T, Hillerich BS, Hammonds J, Nawar A, Stead M, Chowdhury S, Gizzi A, Bonanno J, Seidel R, Almo SC, Minor W, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2-Hacid_dh_C domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 37.08 KDa
Source organism: Klebsiella pneumoniae 342
Expression system: Escherichia coli BL21(DE3)
  • Canonical: B5XVG7 (Residues: 1-310; Coverage: 100%)
Gene name: KPK_1152
Sequence domains: D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 76.567Å b: 76.567Å c: 101.281Å
α: 90° β: 90° γ: 90°
R R work R free
0.183 0.181 0.22
Expression system: Escherichia coli BL21(DE3)