4n0n

X-ray diffraction
2Å resolution

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
ATP + H(2)O = ADP + phosphate
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Helicase Chain: A
Molecule details ›
Chain: A
Length: 423 amino acids
Theoretical weight: 45.66 KDa
Source organism: Equine arteritis virus Bucyrus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19811 (Residues: 2371-2772; Coverage: 13%)
Gene names: 1a-1b, rep
Sequence domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A, BSRF BEAMLINE 1W2B
Spacegroup: P21212
Unit cell:
a: 89.763Å b: 91.041Å c: 57.739Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.224
Expression system: Escherichia coli