› 4msb

X-ray diffraction
1.55Å resolution

RNA 10mer duplex with two 2'-5'-linkages

Released:
DOI: 10.2210/pdb4msb/pdb
PDB 4msb coloured by chain and viewed from the front.
PDB 4msb coloured by chain and viewed from the side.
PDB 4msb coloured by chain and viewed from the top.
Source organism: Synthetic construct
Primary publication:
Structural insights into the effects of 2'-5' linkages on the RNA duplex.
Sheng J, Li L, Engelhart AE, Gan J, Wang J, Szostak JW
Proc. Natl. Acad. Sci. U.S.A. 111 3050-5 (2014)
PMID: 24516151

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA 10mer duplex with two 2'-5'-linkages Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 10 nucleotides
Theoretical weight: 3.21 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SR 7 x SR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1, ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 96.15Å b: 25.893Å c: 69.722Å
α: 90° β: 112.61° γ: 90°
R-values:
R R work R free
0.185 0.182 0.247
Expression system: Not provided

Quick links

Citations

8 review citations

Peptides before and during the nucleotide world: an origins story emphasizing cooperation between proteins and nucleic acids.
Fried et al. (2022)
7 more

6 mentions without citation

Structural insights into the effects of 2'-5' linkages on the RNA duplex.
Sheng et al. (2014)
5 more