4mmz

X-ray diffraction
3.1Å resolution

Integrin AlphaVBeta3 ectodomain bound to an antagonistic tenth domain of Fibronectin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Integrin alpha-V Chain: A
Molecule details ›
Chain: A
Length: 959 amino acids
Theoretical weight: 106.05 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P06756 (Residues: 31-989; Coverage: 94%)
Gene names: ITGAV, MSK8, VNRA, VTNR
Sequence domains:
Structure domains:
Integrin beta-3 Chain: B
Molecule details ›
Chain: B
Length: 692 amino acids
Theoretical weight: 76.52 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P05106 (Residues: 27-718; Coverage: 91%)
Gene names: GP3A, ITGB3
Sequence domains:
Structure domains:
Ugl-Y3 Chain: C
Molecule details ›
Chain: C
Length: 98 amino acids
Theoretical weight: 10.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P02751 (Residues: 1539-1631; Coverage: 4%)
Gene names: FN, FN1
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 129.79Å b: 129.79Å c: 307.676Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.203 0.256
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21