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Structural basis for the inhibition of human lysozyme by PliC from Brucella abortus.

Um SH, Kim JS, Kim K, Kim N, Cho HS, Ha NC
Biochemistry 52 9385-93 (2013)
Related entries: 4mir, 4mis

Cited: 15 times
EuropePMC logo PMID: 24308818

Abstract

Lysozymes are the first line of defense for a diverse range of organisms that catalyze the degradation of bacterial peptidoglycan. Gram-negative bacteria produce proteinaceous lysozyme inhibitors to protect themselves from the action of lysozymes. To date, MliC or PliC (membrane-bound or periplasmic inhibitor of c-type lysozyme, respectively) has been found in various Gram-negative bacteria. Here, we report the crystal structures of Brucella abortus PliC and its complex with human c-type lysozyme. The complex structure demonstrates that the invariant loop of MliC/PliC plays a crucial role in the inhibition of lysozyme via its insertion into the active site cleft of the lysozyme, as previously observed in the complex structure of Pseudomonas aeruginosa MliC and chicken c-type lysozyme. We identified a new binding interface between a loop adjacent to the active site of human lysozyme and a loop carrying Glu112 of B. abortus PliC, the structure of which was disordered in P. aeruginosa MliC. Because MliC/PliC family members have been implicated as putative colonization or virulence factors, the structures and mechanism of action of MliC/PliC will be relevant to the control of bacterial growth in animal hosts.

Articles - 4ml7 mentioned but not cited (3)

  1. Structure of the Neisseria Adhesin Complex Protein (ACP) and its role as a novel lysozyme inhibitor. Humbert MV, Awanye AM, Lian LY, Derrick JP, Christodoulides M. PLoS Pathog. 13 e1006448 (2017)
  2. Structure of the Recombinant Neisseria gonorrhoeae Adhesin Complex Protein (rNg-ACP) and Generation of Murine Antibodies with Bactericidal Activity against Gonococci. Almonacid-Mendoza HL, Humbert MV, Dijokaite A, Cleary DW, Soo Y, Hung MC, Orr CM, Machelett MM, Tews I, Christodoulides M. mSphere 3 (2018)
  3. Characterization of two putative Dichelobacter nodosus footrot vaccine antigens identifies the first lysozyme inhibitor in the genus. Humbert MV, Jackson A, Orr CM, Tews I, Christodoulides M. Sci Rep 9 10055 (2019)


Reviews citing this publication (2)

  1. Interplay between Peptidoglycan Biology and Virulence in Gram-Negative Pathogens. Juan C, Torrens G, Barceló IM, Oliver A. Microbiol. Mol. Biol. Rev. 82 (2018)
  2. Immune Response to Mucosal Brucella Infection. López-Santiago R, Sánchez-Argáez AB, De Alba-Núñez LG, Baltierra-Uribe SL, Moreno-Lafont MC. Front Immunol 10 1759 (2019)

Articles citing this publication (10)

  1. Edwardsiella tarda MliC, a lysozyme inhibitor that participates in pathogenesis in a manner that parallels Ivy. Li MF, Wang C, Sun L. Infect. Immun. 83 583-590 (2015)
  2. Lipoprotein LprI of Mycobacterium tuberculosis Acts as a Lysozyme Inhibitor. Sethi D, Mahajan S, Singh C, Lama A, Hade MD, Gupta P, Dikshit KL. J. Biol. Chem. 291 2938-2953 (2016)
  3. A Moraxella catarrhalis two-component signal transduction system necessary for growth in liquid media affects production of two lysozyme inhibitors. Joslin SN, Pybus C, Labandeira-Rey M, Evans AS, Attia AS, Brautigam CA, Hansen EJ. Infect. Immun. 83 146-160 (2015)
  4. Crystal structure of bacteriophage T4 Spackle as determined by native SAD phasing. Shi K, Kurniawan F, Banerjee S, Moeller NH, Aihara H. Acta Crystallogr D Struct Biol 76 899-904 (2020)
  5. SliC is a surface-displayed lipoprotein that is required for the anti-lysozyme strategy during Neisseria gonorrhoeae infection. Zielke RA, Le Van A, Baarda BI, Herrera MF, Acosta CJ, Jerse AE, Sikora AE. PLoS Pathog. 14 e1007081 (2018)
  6. Structural basis of superinfection exclusion by bacteriophage T4 Spackle. Shi K, Oakland JT, Kurniawan F, Moeller NH, Banerjee S, Aihara H. Commun Biol 3 691 (2020)
  7. Structure and Function of the Autolysin SagA in the Type IV Secretion System of Brucella abortus. Hyun Y, Baek Y, Lee C, Ki N, Ahn J, Ryu S, Ha NC. Mol Cells 44 517-528 (2021)
  8. Integrated analysis of a lncRNA‑mRNA network reveals a potential mechanism underlying necrotizing enterocolitis. Chen W, Yan X, Tian T, Yan R, Wang X, Yu Z, Li Y, Zhang L, Han S. Mol Med Rep 22 423-435 (2020)
  9. PhiA, a Peptidoglycan Hydrolase Inhibitor of Brucella Involved in the Virulence Process. Del Giudice MG, Romani AM, Ugalde JE, Czibener C. Infect. Immun. 87 (2019)
  10. The structure of the proteinaceous inhibitor PliI from Aeromonas hydrophila in complex with its target lysozyme. Leysen S, Van Herreweghe JM, Yoneda K, Ogata M, Usui T, Araki T, Michiels CW, Strelkov SV. Acta Crystallogr. D Biol. Crystallogr. 71 344-351 (2015)


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