4mjt

X-ray diffraction
2.85Å resolution

Crystal structure of the oligomeric pore-forming toxin pro-Monalysin

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172891 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Monalysin Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 236 amino acids
Theoretical weight: 26.46 KDa
Source organism: Pseudomonas entomophila L48
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1I8U1 (Residues: 36-271; Coverage: 87%)
Gene names: PSEEN3174, mnl
Sequence domains: Beta barrel Pore-forming domain
Monalysin Chains: J, K, L, M, N, O, P, Q, R
Molecule details ›
Chains: J, K, L, M, N, O, P, Q, R
Length: 27 amino acids
Theoretical weight: 2.89 KDa
Source organism: Pseudomonas entomophila L48
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1I8U1 (Residues: 9-35; Coverage: 10%)
Gene names: PSEEN3174, mnl

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 162.4Å b: 146.2Å c: 144.32Å
α: 90° β: 122.8° γ: 90°
R-values:
R R work R free
0.184 0.182 0.216
Expression system: Escherichia coli