› 4miw

X-ray diffraction
1.72Å resolution

High-resolution structure of the N-terminal endonuclease domain of the Lassa virus L polymerase

Released:
DOI: 10.2210/pdb4miw/pdb
PDB entry 4miw coloured by chain, front view.
PDB entry 4miw coloured by chain, side view.
PDB entry 4miw coloured by chain, top view.
Source organism: Mammarenavirus lassaense
Primary publication:
High-resolution structure of the N-terminal endonuclease domain of the Lassa virus L polymerase in complex with magnesium ions.
Wallat GD, Huang Q, Wang W, Dong H, Ly H, Liang Y, Dong C
PLoS One 9 e87577 (2014)
PMID: 24516554

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase L Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 20.31 KDa
Source organism: Mammarenavirus lassaense
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6GWS2 (Residues: 1-170; Coverage: 8%)
Gene name: L
Sequence domains: Arenavirus cap snatching domain
Structure domains: Arenavirus RNA polymerase

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P43212
Unit cell:
a: 57.72Å b: 57.72Å c: 134.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.178
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Transcription and replication mechanisms of Bunyaviridae and Arenaviridae L proteins.
Ferron et al. (2017)
4 more

5 mentions without citation

Comparative Structural and Functional Analysis of Bunyavirus and Arenavirus Cap-Snatching Endonucleases.
Reguera et al. (2016)
4 more