4mi2

X-ray diffraction
2.3Å resolution

Crystal structure of putative Enoyl-CoA hydratase/isomerase from Mycobacterium abscessus

Released:
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA = 2-oxepin-2(3H)-ylideneacetyl-CoA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative enoyl-CoA hydratase/isomerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 267 amino acids
Theoretical weight: 27.37 KDa
Source organism: Mycobacteroides abscessus ATCC 19977
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1MJ43 (Residues: 1-267; Coverage: 100%)
Gene name: MAB_0905
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P41212
Unit cell:
a: 94.92Å b: 94.92Å c: 195.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.213
Expression system: Escherichia coli BL21(DE3)