› 4m83

X-ray diffraction
1.7Å resolution

Ensemble refinement of protein crystal structure (2IYF) of macrolide glycosyltransferases OleD complexed with UDP and Erythromycin A

Released:
DOI: 10.2210/pdb4m83/pdb
PDB 4m83 coloured by chain and viewed from the front.
PDB 4m83 coloured by chain and viewed from the side.
PDB 4m83 coloured by chain and viewed from the top.
Source organism: Streptomyces antibioticus
Entry authors: Wang F, Helmich KE, Xu W, Singh S, Olmos Jr JL, Martinez iii E, Bingman CA, Thorson JS, Phillips Jr GN, Enzyme Discovery for Natural Product Biosynthesis (NatPro)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oleandomycin glycosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 415 amino acids
Theoretical weight: 45.37 KDa
Source organism: Streptomyces antibioticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53685 (Residues: 1-415; Coverage: 97%)
Gene names: UGT102A2, oleD
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

3 bound ligands:
Ligand ERY 2 x ERY
Ligand UDP 2 x UDP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 72.718Å b: 65.778Å c: 91.94Å
α: 90° β: 100.42° γ: 90°
R-values:
R R work R free
0.161 0.16 0.201
Expression system: Escherichia coli

Quick links