4m83

X-ray diffraction
1.7Å resolution

Ensemble refinement of protein crystal structure (2IYF) of macrolide glycosyltransferases OleD complexed with UDP and Erythromycin A

Released:
Source organism: Streptomyces antibioticus
Entry authors: Wang F, Helmich KE, Xu W, Singh S, Olmos Jr JL, Martinez iii E, Bingman CA, Thorson JS, Phillips Jr GN, Enzyme Discovery for Natural Product Biosynthesis (NatPro)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oleandomycin glycosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 415 amino acids
Theoretical weight: 45.37 KDa
Source organism: Streptomyces antibioticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53685 (Residues: 1-415; Coverage: 97%)
Gene names: UGT102A2, oleD
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 72.718Å b: 65.778Å c: 91.94Å
α: 90° β: 100.42° γ: 90°
R-values:
R R work R free
0.161 0.16 0.201
Expression system: Escherichia coli