› 4m4s

X-ray diffraction
2.25Å resolution

Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in complex with GDP and formate ion mimic aIF2gamma*GDP*Pi complex (a formate ion substitutes for Pi)

Released:
DOI: 10.2210/pdb4m4s/pdb
PDB entry 4m4s coloured by chain, front view.
PDB entry 4m4s coloured by chain, side view.
PDB entry 4m4s coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Conformational transitions in the γ subunit of the archaeal translation initiation factor 2.
Nikonov O, Stolboushkina E, Arkhipova V, Kravchenko O, Nikonov S, Garber M
Acta Crystallogr D Biol Crystallogr 70 658-67 (2014)
PMID: 24598735

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translation initiation factor 2 subunit gamma Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 45.63 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q980A5 (Residues: 1-415; Coverage: 100%)
Gene names: SSO0412, eif2g
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand FMT 29 x FMT
Ligand GDP 1 x GDP
Ligand MG 2 x MG
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: R32
Unit cell:
a: 142.86Å b: 142.86Å c: 218.41Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.152 0.182
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Start Codon Recognition in Eukaryotic and Archaeal Translation Initiation: A Common Structural Core.
Schmitt et al. (2019)
2 other articles