4m0a

X-ray diffraction
1.85Å resolution

Human DNA Polymerase Mu post-catalytic complex

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA-directed DNA/RNA polymerase mu Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 40.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NP87 (Residues: 132-397, 411-494; Coverage: 71%)
Gene names: POLM, polmu
Sequence domains:
Structure domains:
template strand Chain: T
Molecule details ›
Chain: T
Length: 9 nucleotides
Theoretical weight: 2.74 KDa
upstream primer strand Chain: P
Molecule details ›
Chain: P
Length: 5 nucleotides
Theoretical weight: 1.5 KDa
downstream primer strand Chain: D
Molecule details ›
Chain: D
Length: 4 nucleotides
Theoretical weight: 1.19 KDa

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 60.018Å b: 68.67Å c: 110.444Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.163 0.2
Expression system: Escherichia coli