› 4lv5

X-ray diffraction
1.7Å resolution

Murine IRGa6 bound to Toxoplasma ROP5B, a pseudokinase GDI

Released:
DOI: 10.2210/pdb4lv5/pdb
PDB entry 4lv5 coloured by chain, front view.
PDB entry 4lv5 coloured by chain, side view.
PDB entry 4lv5 coloured by chain, top view.
Source organisms:
Entry authors: Reese ML, Boothroyd JC

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-123430 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein kinase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 371 amino acids
Theoretical weight: 41.22 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F2YGR7 (Residues: 175-541; Coverage: 70%)
Gene names: ROP5, ROP5B, TGRH88_057710
Sequence domains: Kinase-like
Structure domains:
Interferon-inducible GTPase 1 Chain: B
Molecule details ›
Chain: B
Length: 423 amino acids
Theoretical weight: 48.48 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9QZ85 (Residues: 1-413; Coverage: 100%)
Gene names: Iigp1, Irga6
Sequence domains: Interferon-inducible GTPase (IIGP)
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand ADP 1 x ADP
Ligand BME 1 x BME
Ligand EDO 6 x EDO
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P1
Unit cell:
a: 52.6Å b: 54.7Å c: 85.6Å
α: 99° β: 106.6° γ: 106.9°
R-values:
R R work R free
0.194 0.192 0.228
Expression system: Escherichia coli BL21(DE3)

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