› 4lsr

X-ray diffraction
2.28Å resolution

Crystal structure of broadly and potently neutralizing antibody VRC-CH31 in complex with HIV-1 clade A/E stran 93TH057 gp120 with LOOP D and Loop V5 from clade A strain KER_2018_11

Released:
DOI: 10.2210/pdb4lsr/pdb
PDB entry 4lsr coloured by chain, front view.
PDB entry 4lsr coloured by chain, side view.
PDB entry 4lsr coloured by chain, top view.
Source organisms:
Primary publication:
Multidonor analysis reveals structural elements, genetic determinants, and maturation pathway for HIV-1 neutralization by VRC01-class antibodies.
Zhou T, Zhu J, Wu X, Moquin S, Zhang B, Acharya P, Georgiev IS, Altae-Tran HR, Chuang GY, Joyce MG, Kwon YD, Longo NS, Louder MK, Luongo T, McKee K, Schramm CA, Skinner J, Yang Y, Yang Z, Zhang Z, Zheng A, Bonsignori M, Haynes BF, Scheid JF, Nussenzweig MC, Simek M, Burton DR, Koff WC, Mullikin JC, Connors M, Shapiro L, Nabel GJ, Mascola JR, Kwong PD
Immunity 39 245-58 (2013)
PMID: 23911655

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211975 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Envelope glycoprotein gp160 Chain: G
Molecule details ›
Chain: G
Length: 356 amino acids
Theoretical weight: 39.56 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q0ED31 (Residues: 43-454, 455-486; Coverage: 41%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
HEAVY CHAIN OF ANTIBODY VRC-CH31 Chain: H
Molecule details ›
Chain: H
Length: 236 amino acids
Theoretical weight: 25.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
LIGHT CHAIN OF ANTIBODY VRC-CH31 (N70D MUTATION) Chain: L
Molecule details ›
Chain: L
Length: 210 amino acids
Theoretical weight: 22.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand NAG 9 x NAG
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 64.934Å b: 67.726Å c: 220.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.194 0.258
Expression system: Homo sapiens

Quick links

Citations

58 review citations

The promise and challenge of high-throughput sequencing of the antibody repertoire.
Georgiou et al. (2014)
57 more

3 mentions without citation

Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design.
Burton et al. (2016)
2 more