4lsr

X-ray diffraction
2.28Å resolution

Crystal structure of broadly and potently neutralizing antibody VRC-CH31 in complex with HIV-1 clade A/E stran 93TH057 gp120 with LOOP D and Loop V5 from clade A strain KER_2018_11

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Envelope glycoprotein gp160 Chain: G
Molecule details ›
Chain: G
Length: 356 amino acids
Theoretical weight: 39.56 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q0ED31 (Residues: 43-454, 455-486; Coverage: 41%)
Gene name: env
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
HEAVY CHAIN OF ANTIBODY VRC-CH31 Chain: H
Molecule details ›
Chain: H
Length: 236 amino acids
Theoretical weight: 25.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
LIGHT CHAIN OF ANTIBODY VRC-CH31 (N70D MUTATION) Chain: L
Molecule details ›
Chain: L
Length: 210 amino acids
Theoretical weight: 22.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 64.934Å b: 67.726Å c: 220.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.194 0.258
Expression system: Homo sapiens