› 4lrk

X-ray diffraction
2.27Å resolution

Bacterial Effector NleH2 Kinase Domain

Released:
DOI: 10.2210/pdb4lrk/pdb
PDB entry 4lrk coloured by chain, front view.
PDB entry 4lrk coloured by chain, side view.
PDB entry 4lrk coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
NleH defines a new family of bacterial effector kinases.
Grishin AM, Cherney M, Anderson DH, Phanse S, Babu M, Cygler M
Structure 22 250-9 (2014)
PMID: 24373767

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-186870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
T3SS secreted effector NleH Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 167 amino acids
Theoretical weight: 18.7 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8XAL6 (Residues: 140-303; Coverage: 54%)
Gene names: ECs_1814, nleH1_2
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P4212
Unit cell:
a: 145.288Å b: 145.288Å c: 83.456Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The Genetics of Enteropathogenic Escherichia coli Virulence.
Pearson et al. (2016)
4 more

2 mentions without citation

Structural insight into effector proteins of Gram-negative bacterial pathogens that modulate the phosphoproteome of their host.
Grishin et al. (2015)
1 more