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Crystal structure of a Cdc6 phosphopeptide in complex with Cks1

X-ray diffraction
2.9Å resolution
PDB entry 4lpa coloured by chain, front view.
PDB entry 4lpa coloured by chain, side view.
PDB entry 4lpa coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Assemblies composition:
monomeric (preferred)
homo tetramer
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Cyclin-dependent kinases regulatory subunit
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Multimeric state: monomeric
Accessible surface area: 7804.56 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148993
Assembly 2
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Assembly Name: Cyclin-dependent kinases regulatory subunit
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Multimeric state: monomeric
Accessible surface area: 7718.68 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148993
Assembly 3
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Assembly Name: Cyclin-dependent kinases regulatory subunit
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Multimeric state: monomeric
Accessible surface area: 7751.79 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148993
Assembly 4
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Assembly Name: Cyclin-dependent kinases regulatory subunit
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Multimeric state: monomeric
Accessible surface area: 7814.89 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148993
Assembly 5
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Assembly Name: Cyclin-dependent kinases regulatory subunit
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Multimeric state: homo tetramer
Accessible surface area: 25023.62 Å2
Buried surface area: 6057.85 Å2
Dissociation area: 1,758.09 Å2
Dissociation energy (ΔGdiss): 12.57 kcal/mol
Dissociation entropy (TΔSdiss): 13.3 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-148995

Macromolecules

Cyclin-dependent kinases regulatory subunit
Chains: A, B, C, D
Length: 119 amino acids
Theoretical weight: 14.26 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20486 (Residues: 1-113; Coverage: 75%)
Gene names: CKS1, YBR1011, YBR135W
Pfam: Cyclin-dependent kinase regulatory subunit
InterPro:
CATH: Cyclin-dependent kinase, regulatory subunit
Cyclin-dependent kinases regulatory subunit in PDB entry 4lpa, assembly 1, front view.
Cyclin-dependent kinases regulatory subunit in PDB entry 4lpa, assembly 1, side view.
Cyclin-dependent kinases regulatory subunit in PDB entry 4lpa, assembly 1, top view.
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