X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF purine nucleoside phosphorylase I from Spirosoma linguale DSM 74, NYSGRC Target 029362

Source organism: Spirosoma linguale DSM 74
Entry authors: Malashkevich VN, Bonanno JB, Bhosle R, Toro R, Hillerich B, Gizzi A, Garforth S, Kar A, Chan MK, Lafluer J, Patel H, Matikainen B, Chamala S, Lim S, Celikgil A, Villegas G, Evans B, Love J, Fiser A, Khafizov K, Seidel R, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed:
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 33.05 KDa
Source organism: Spirosoma linguale DSM 74
Expression system: Escherichia coli
  • Canonical: D2QIK7 (Residues: 1-271; Coverage: 100%)
Gene name: Slin_3943
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P4332
Unit cell:
a: 125.561Å b: 125.561Å c: 125.561Å
α: 90° β: 90° γ: 90°
R R work R free
0.174 0.172 0.216
Expression system: Escherichia coli