4lmb

X-ray diffraction
1.91Å resolution

Crystal structure analysis of O-acetylserine sulfhydrylase CysK2 complexed with cystine from Microcystis aeruginosa 7806

Released:

Function and Biology Details

Reaction catalysed:
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine synthase Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 34.74 KDa
Source organism: Microcystis aeruginosa PCC 7806
Expression system: Escherichia coli
UniProt:
  • Canonical: A8YBP8 (Residues: 1-315; Coverage: 100%)
Gene name: IPF_3084
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6522
Unit cell:
a: 99.846Å b: 99.846Å c: 164.141Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.202 0.23
Expression system: Escherichia coli