4ll8

X-ray diffraction
3.58Å resolution

Complex of carboxy terminal domain of Myo4p and She3p middle fragment

Released:
Primary publication:
Structure of a myosin•adaptor complex and pairing by cargo.
Proc. Natl. Acad. Sci. U.S.A. 111 E1082-90 (2014)
PMID: 24522109

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Myosin-4 Chain: A
Molecule details ›
Chain: A
Length: 539 amino acids
Theoretical weight: 61.56 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32492 (Residues: 918-1471; Coverage: 37%)
Gene names: FUN22, MYO4, SHE1, YAL029C
Sequence domains: DIL domain
SWI5-dependent HO expression protein 3 Chains: B, E
Molecule details ›
Chains: B, E
Length: 235 amino acids
Theoretical weight: 27.17 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38272 (Residues: 81-311; Coverage: 54%)
Gene names: SHE3, YBR1005, YBR130C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 272.013Å b: 38.116Å c: 183.991Å
α: 90° β: 125.24° γ: 90°
R-values:
R R work R free
0.224 0.224 0.268
Expression system: Escherichia coli