4lk5

X-ray diffraction
2.5Å resolution

Crystal structure of a enoyl-CoA hydratase from Mycobacterium avium subsp. paratuberculosis K-10

Released:
Entry authors: Kumaran D, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, Lafleur J, Seidel R, Villigas G, Zencheck W, Al Obaidi N, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EchA11 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 292 amino acids
Theoretical weight: 30.62 KDa
Source organism: Mycobacterium avium subsp. paratuberculosis K-10
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q73WM1 (Residues: 1-270; Coverage: 100%)
Gene names: MAP_2639, echA11
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3221
Unit cell:
a: 78.328Å b: 78.328Å c: 212.933Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.257 0.199 0.258
Expression system: Escherichia coli BL21(DE3)