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Intermediates in the guanine nucleotide exchange reaction of Rab8 protein catalyzed by guanine nucleotide exchange factors Rabin8 and GRAB.

Guo Z, Hou X, Goody RS, Itzen A
J Biol Chem 288 32466-32474 (2013)
Related entries: 4lhv, 4lhx, 4lhy, 4lhz, 4li0

Cited: 41 times
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Abstract

Small G-proteins of the Ras superfamily control the temporal and spatial coordination of intracellular signaling networks by acting as molecular on/off switches. Guanine nucleotide exchange factors (GEFs) regulate the activation of these G-proteins through catalytic replacement of GDP by GTP. During nucleotide exchange, three distinct substrate·enzyme complexes occur: a ternary complex with GDP at the start of the reaction (G-protein·GEF·GDP), an intermediary nucleotide-free binary complex (G-protein·GEF), and a ternary GTP complex after productive G-protein activation (G-protein·GEF·GTP). Here, we show structural snapshots of the full nucleotide exchange reaction sequence together with the G-protein substrates and products using Rabin8/GRAB (GEF) and Rab8 (G-protein) as a model system. Together with a thorough enzymatic characterization, our data provide a detailed view into the mechanism of Rabin8/GRAB-mediated nucleotide exchange.

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  2. Intermediates in the guanine nucleotide exchange reaction of Rab8 protein catalyzed by guanine nucleotide exchange factors Rabin8 and GRAB. Guo Z, Guo Z, Hou X, Goody RS, Itzen A. J Biol Chem 288 32466-32474 (2013)
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  2. Molecular control of Rab activity by GEFs, GAPs and GDI. Müller MP, Goody RS. Small GTPases 9 5-21 (2018)
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  2. Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1. Lai YC, Kondapalli C, Lehneck R, Procter JB, Dill BD, Woodroof HI, Gourlay R, Peggie M, Macartney TJ, Corti O, Corvol JC, Campbell DG, Itzen A, Trost M, Muqit MM. EMBO J 34 2840-2861 (2015)
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  5. SCD1 and SCD2 Form a Complex That Functions with the Exocyst and RabE1 in Exocytosis and Cytokinesis. Mayers JR, Hu T, Wang C, Cárdenas JJ, Tan Y, Pan J, Bednarek SY. Plant Cell 29 2610-2625 (2017)
  6. Mutations in LRRK2 linked to Parkinson disease sequester Rab8a to damaged lysosomes and regulate transferrin-mediated iron uptake in microglia. Mamais A, Kluss JH, Bonet-Ponce L, Landeck N, Langston RG, Smith N, Beilina A, Kaganovich A, Ghosh MC, Pellegrini L, Kumaran R, Papazoglou I, Heaton GR, Bandopadhyay R, Maio N, Kim C, LaVoie MJ, Gershlick DC, Cookson MR. PLoS Biol 19 e3001480 (2021)
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  9. Structural outline of the detailed mechanism for elongation factor Ts-mediated guanine nucleotide exchange on elongation factor Tu. Thirup SS, Van LB, Nielsen TK, Knudsen CR. J Struct Biol 191 10-21 (2015)
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  12. Inferring joint sequence-structural determinants of protein functional specificity. Neuwald AF, Aravind L, Altschul SF. Elife 7 e29880 (2018)
  13. Rabin8 suppresses autophagosome formation independently of its guanine nucleotide-exchange activity towards Rab8. Amagai Y, Itoh T, Fukuda M, Mizuno K. J Biochem 158 139-153 (2015)
  14. Guanine nucleotide exchange factors activate Rab8a for Toll-like receptor signalling. Tong SJ, Wall AA, Hung Y, Luo L, Stow JL. Small GTPases 12 27-43 (2021)
  15. Inositol Pyrophosphate Metabolism Regulates Presynaptic Vesicle Cycling at Central Synapses. Park SJ, Park H, Kim MG, Zhang S, Park SE, Kim S, Chung C. iScience 23 101000 (2020)
  16. Structural basis of guanine nucleotide exchange for Rab11 by SH3BP5. Goto-Ito S, Morooka N, Yamagata A, Sato Y, Sato K, Fukai S. Life Sci Alliance 2 e201900297 (2019)
  17. Tankyrase regulates epithelial lumen formation via suppression of Rab11 GEFs. Chandrakumar AA, Coyaud É, Marshall CB, Ikura M, Raught B, Rottapel R. J Cell Biol 220 e202008037 (2021)
  18. Crystal structure of tetrameric human Rabin8 GEF domain. Vetter M, Boegholm N, Christensen A, Bhogaraju S, Andersen MB, Lorentzen A, Lorentzen E. Proteins 86 405-413 (2018)
  19. Crystal structure of the GDP-bound GTPase domain of Rab5a from Leishmania donovani. Zohib M, Maheshwari D, Pal RK, Freitag-Pohl S, Biswal BK, Pohl E, Arora A. Acta Crystallogr F Struct Biol Commun 76 544-556 (2020)
  20. Protein quality control machinery supports primary ciliogenesis by eliminating GDP-bound Rab8-family GTPases. Takahashi T, Shirai J, Matsuda M, Nakanaga S, Matsushita S, Wakita K, Hayashishita M, Suzuki R, Noguchi A, Yokota N, Kawahara H. iScience 26 106652 (2023)


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