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› 4lhw

X-ray diffraction
1.55Å resolution

Crystal structure of Rab8 in its active GppNHp-bound form

Released:
DOI: 10.2210/pdb4lhw/pdb
PDB entry 4lhw coloured by chain, front view.
PDB entry 4lhw coloured by chain, side view.
PDB entry 4lhw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Intermediates in the guanine nucleotide exchange reaction of Rab8 protein catalyzed by guanine nucleotide exchange factors Rabin8 and GRAB.
Guo Z, Hou X, Goody RS, Itzen A
J Biol Chem 288 32466-32474 (2013)
PMID: 24072714

Function and Biology Details

Reaction catalysed: 
GTP + H2O = GDP + phosphate + H(+).
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158163 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-8A Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 174 amino acids
Theoretical weight: 19.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61006 (Residues: 6-176; Coverage: 83%)
Gene names: MEL, RAB8, RAB8A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 5 x MG
Ligand GNP 5 x GNP
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 90.381Å b: 96.676Å c: 105.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.144 0.183
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Rab proteins and the compartmentalization of the endosomal system.
Wandinger-Ness et al. (2014)
3 more

17 mentions without citation

Rab GTPases and their interacting protein partners: Structural insights into Rab functional diversity.
Pylypenko et al. (2018)
16 more