4lhw

X-ray diffraction
1.55Å resolution

Crystal structure of Rab8 in its active GppNHp-bound form

Released:

Function and Biology Details

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-8A Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 174 amino acids
Theoretical weight: 19.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61006 (Residues: 6-176; Coverage: 83%)
Gene names: MEL, RAB8, RAB8A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 90.381Å b: 96.676Å c: 105.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.144 0.183
Expression system: Escherichia coli BL21