› 4lhw

X-ray diffraction
1.55Å resolution

Crystal structure of Rab8 in its active GppNHp-bound form

Released:
DOI: 10.2210/pdb4lhw/pdb
PDB 4lhw coloured by chain and viewed from the front.
PDB 4lhw coloured by chain and viewed from the side.
PDB 4lhw coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Intermediates in the Guanine Nucleotide Exchange Reaction of Rab8 Catalyzed by Rabin8/GRAB.
Guo Z, Hou X, Goody RS, Itzen A
J. Biol. Chem. (2013)
PMID: 24072714

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-8A Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 174 amino acids
Theoretical weight: 19.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61006 (Residues: 6-176; Coverage: 83%)
Gene names: MEL, RAB8, RAB8A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 5 x MG
Ligand GNP 5 x GNP
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 90.381Å b: 96.676Å c: 105.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.144 0.183
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Structural Insights into the Regulation Mechanism of Small GTPases by GEFs.
Toma-Fukai et al. (2019)
3 more

9 mentions without citation

Rab GTPases and their interacting protein partners: Structural insights into Rab functional diversity.
Pylypenko et al. (2018)
8 more