4lhc

X-ray diffraction
1.9Å resolution

Crystal structure of Synechocystis sp. PCC 6803 glycine decarboxylase (P-protein), holo form with pyridoxal-5'-phosphate and glycine

Released:

Function and Biology Details

Reaction catalysed:
Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO(2)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine dehydrogenase (decarboxylating) Chains: A, B
Molecule details ›
Chains: A, B
Length: 983 amino acids
Theoretical weight: 107.65 KDa
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Expression system: Escherichia coli
UniProt:
  • Canonical: P74416 (Residues: 1-983; Coverage: 100%)
Gene names: gcvP, slr0293
Sequence domains: Glycine cleavage system P-protein

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P321
Unit cell:
a: 160.05Å b: 160.05Å c: 159.833Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.155 0.186
Expression system: Escherichia coli