› 4lbx

X-ray diffraction
1.7Å resolution

Crystal structure of probable sugar kinase protein from Rhizobium Etli CFN 42 complexed with cytidine

Released:
DOI: 10.2210/pdb4lbx/pdb
PDB 4lbx coloured by chain and viewed from the front.
PDB 4lbx coloured by chain and viewed from the side.
PDB 4lbx coloured by chain and viewed from the top.
Source organism: Rhizobium etli CFN 42
Entry authors: Malashkevich VN, Bhosle R, Toro R, Hillerich B, Gizzi A, Garforth S, Kar A, Chan MK, Lafluer J, Patel H, Matikainen B, Chamala S, Lim S, Celikgil A, Villegas G, Evans B, Love J, Fiser A, Khafizov K, Seidel R, Bonanno JB, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PfkB domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 352 amino acids
Theoretical weight: 38.85 KDa
Source organism: Rhizobium etli CFN 42
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2KDX6 (Residues: 1-330; Coverage: 100%)
Gene name: RHE_CH00135
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

4 bound ligands:
Ligand ADN 2 x ADN
Ligand CTN 2 x CTN
Ligand DMS 4 x DMS
Ligand K 2 x K
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 80.878Å b: 90.809Å c: 92.155Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.237
Expression system: Escherichia coli BL21(DE3)

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