4lad

X-ray diffraction
2.3Å resolution

Crystal Structure of the Ube2g2:RING-G2BR complex

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin-conjugating enzyme E2 G2 Chain: A
Molecule details ›
Chain: A
Length: 165 amino acids
Theoretical weight: 18.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P60604 (Residues: 1-165; Coverage: 100%)
Gene names: UBC7, UBE2G2
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme
E3 ubiquitin-protein ligase AMFR Chain: B
Molecule details ›
Chain: B
Length: 150 amino acids
Theoretical weight: 15.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9UKV5 (Residues: 313-393, 574-600; Coverage: 17%)
Gene names: AMFR, RNF45
Sequence domains:
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P41212
Unit cell:
a: 58.246Å b: 58.246Å c: 158.431Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.226 0.281
Expression system: Escherichia coli BL21