› 4l85

X-ray diffraction
2.2Å resolution

Crystal structure of receiver domain of KdpE D52A mutant from E. coli

Released:
DOI: 10.2210/pdb4l85/pdb
PDB entry 4l85 coloured by chain, front view.
PDB entry 4l85 coloured by chain, side view.
PDB entry 4l85 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
An asymmetric heterodomain interface stabilizes a response regulator-DNA complex.
Narayanan A, Kumar S, Evrard AN, Paul LN, Yernool DA
Nat Commun 5 3282 (2014)
PMID: 24526190

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
KDP operon transcriptional regulatory protein KdpE Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 123 amino acids
Theoretical weight: 13.49 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P21866 (Residues: 3-121; Coverage: 53%)
Gene names: JW5096, b0694, kdpE
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand IOD 35 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P3121
Unit cell:
a: 108.918Å b: 108.918Å c: 74.408Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.219
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
1 more