› 4l6h

X-ray diffraction
1.75Å resolution

Crystal structure of the Candida albicans Methionine Synthase in complex with Methotrexate and Homocysteine

Released:
DOI: 10.2210/pdb4l6h/pdb
PDB entry 4l6h coloured by chain, front view.
PDB entry 4l6h coloured by chain, side view.
PDB entry 4l6h coloured by chain, top view.
Source organism: Candida albicans SC5314
Primary publication:
Structural analysis of a fungal methionine synthase with substrates and inhibitors.
Ubhi D, Kago G, Monzingo AF, Robertus JD
J Mol Biol 426 1839-47 (2014)
PMID: 24524835

Function and Biology Details

Reaction catalysed: 
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 789 amino acids
Theoretical weight: 88.19 KDa
Source organism: Candida albicans SC5314
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P82610 (Residues: 1-767; Coverage: 100%)
Gene names: CAALFM_CR01620CA, CaO19.10083, CaO19.2551, MET6
Sequence domains:
Structure domains: TIM Barrel

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand HCS 1 x HCS
Ligand MTX 1 x MTX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P212121
Unit cell:
a: 77.068Å b: 98.981Å c: 101.936Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.235
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Molecular targets for antifungals in amino acid and protein biosynthetic pathways.
Kuplińska et al. (2021)
5 other articles