› 4l69

X-ray diffraction
2.9Å resolution

Rv2372c of Mycobacterium tuberculosis is RsmE like methyltransferse

Released:
DOI: 10.2210/pdb4l69/pdb
PDB entry 4l69 coloured by chain, front view.
PDB entry 4l69 coloured by chain, side view.
PDB entry 4l69 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
The structure of Rv2372c identifies an RsmE-like methyltransferase from Mycobacterium tuberculosis.
Kumar A, Kumar S, Taneja B
Acta Crystallogr D Biol Crystallogr 70 821-32 (2014)
PMID: 24598751

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase E Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 27.77 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGX1 (Residues: 1-262; Coverage: 100%)
Gene names: MTCY27.08, Rv2372c, rsmE
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P41212
Unit cell:
a: 81.94Å b: 81.94Å c: 115.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.236
Expression system: Escherichia coli

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Citations

1 review citation

16S rRNA Methyltransferases as Novel Drug Targets Against Tuberculosis.
Salaikumaran et al. (2022)
6 other articles