› 4l4u

X-ray diffraction
2.2Å resolution

Crystal structure of construct containing A. aeolicus NtrC1 receiver, central and DNA binding domains

Released:
DOI: 10.2210/pdb4l4u/pdb
PDB entry 4l4u coloured by chain, front view.
PDB entry 4l4u coloured by chain, side view.
PDB entry 4l4u coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Structure, function, and tethering of DNA-binding domains in σ⁵⁴ transcriptional activators.
Vidangos N, Maris AE, Young A, Hong E, Pelton JG, Batchelor JD, Wemmer DE
Biopolymers 99 1082-96 (2013)
PMID: 23818155

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator (NtrC family) Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 51.23 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67198 (Residues: 1-439; Coverage: 100%)
Gene names: aq_1117, ntrC1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 102.421Å b: 107.01Å c: 98.181Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.248
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Bacterial Enhancer Binding Proteins-AAA+ Proteins in Transcription Activation.
Gao et al. (2020)
10 other articles

1 mention without citation

Structure, function, and tethering of DNA-binding domains in σ⁵⁴ transcriptional activators.
Vidangos et al. (2013)