› 4l40

X-ray diffraction
2.5Å resolution

Structure of the P450 OleT with a C20 fatty acid substrate bound

Released:
DOI: 10.2210/pdb4l40/pdb
PDB entry 4l40 coloured by chain, front view.
PDB entry 4l40 coloured by chain, side view.
PDB entry 4l40 coloured by chain, top view.
Source organism: Jeotgalicoccus sp. ATCC 8456
Primary publication:
Structure and biochemical properties of the alkene producing cytochrome P450 OleTJE (CYP152L1) from the Jeotgalicoccus sp. 8456 bacterium.
Belcher J, McLean KJ, Matthews S, Woodward LS, Fisher K, Rigby SEJ, Nelson DR, Potts D, Baynham MT, Parker DA, Leys D, Munro AW
J Biol Chem 289 6535-6550 (2014)
PMID: 24443585

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 48.43 KDa
Source organism: Jeotgalicoccus sp. ATCC 8456
Expression system: Escherichia coli
UniProt:
  • Canonical: E9NSU2 (Residues: 1-422; Coverage: 100%)
Gene name: JEM45_03820
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand DCR 1 x DCR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P43212
Unit cell:
a: 59.63Å b: 59.63Å c: 244.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.195 0.269
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Design and engineering of artificial oxygen-activating metalloenzymes.
Nastri et al. (2016)
10 more

16 mentions without citation

Oxygen Activation and Radical Transformations in Heme Proteins and Metalloporphyrins.
Huang et al. (2018)
15 more