X-ray diffraction
2.97Å resolution

Crystal structure of delta516-525 human cystathionine beta-synthase containing C-terminal 6xHis-tag


Function and Biology Details

Reaction catalysed:
L-serine + L-homocysteine = L-cystathionine + H(2)O
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Cystathionine beta-synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 549 amino acids
Theoretical weight: 60.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P35520 (Residues: 1-551; Coverage: 98%)
Gene name: CBS
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand PLP 2 x PLP

Cofactor: Ligand HEM 2 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: I222
Unit cell:
a: 124.365Å b: 136.203Å c: 169.307Å
α: 90° β: 90° γ: 90°
R R work R free
0.242 0.24 0.284
Expression system: Escherichia coli BL21(DE3)