› 4kxa

X-ray diffraction
2.4Å resolution

Crystal structure of human aminopeptidase A complexed with aspartate and calcium

Released:
DOI: 10.2210/pdb4kxa/pdb
PDB entry 4kxa coloured by chain, front view.
PDB entry 4kxa coloured by chain, side view.
PDB entry 4kxa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into central hypertension regulation by human aminopeptidase A.
Yang Y, Liu C, Lin YL, Li F
J Biol Chem 288 25638-25645 (2013)
PMID: 23888046

Function and Biology Details

Reaction catalysed: 
Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glutamyl aminopeptidase Chain: A
Molecule details ›
Chain: A
Length: 888 amino acids
Theoretical weight: 102.72 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q07075 (Residues: 76-957; Coverage: 92%)
Gene name: ENPEP
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand CA 1 x CA
Ligand ZN 1 x ZN
Ligand NAG 2 x NAG
Ligand ASP 1 x ASP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P6422
Unit cell:
a: 142.093Å b: 142.093Å c: 237.414Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.152 0.224
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Novel Therapeutic Approaches Targeting the Renin-Angiotensin System and Associated Peptides in Hypertension and Heart Failure.
Arendse et al. (2019)
3 more

3 mentions without citation

M1 aminopeptidases as drug targets: broad applications or therapeutic niche?
Drinkwater et al. (2017)
2 more