› 4kuo

X-ray diffraction
2Å resolution

A superfast recovering full-length LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae (Photoexcited state)

Released:
DOI: 10.2210/pdb4kuo/pdb
PDB 4kuo coloured by chain and viewed from the front.
PDB 4kuo coloured by chain and viewed from the side.
PDB 4kuo coloured by chain and viewed from the top.
Source organism: Dinoroseobacter shibae DFL 12 = DSM 16493
Primary publication:
Structure and function of a short LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae.
Endres S, Granzin J, Circolone F, Stadler A, Krauss U, Drepper T, Svensson V, Knieps-Grünhagen E, Wirtz A, Cousin A, Tielen P, Willbold D, Jaeger KE, Batra-Safferling R
OpenAccess logo BMC Microbiol 15 30 (2015)
PMID: 25887755

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative blue-light photoreceptor Chain: A
Molecule details ›
Chain: A
Length: 146 amino acids
Theoretical weight: 16.86 KDa
Source organism: Dinoroseobacter shibae DFL 12 = DSM 16493
Expression system: Escherichia coli
UniProt:
  • Canonical: A8LP63 (Residues: 2-139; Coverage: 99%)
Gene name: Dshi_2006
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand RBF 1 x RBF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 90.78Å b: 30.936Å c: 49.538Å
α: 90° β: 113.09° γ: 90°
R-values:
R R work R free
0.164 0.163 0.182
Expression system: Escherichia coli

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Citations

1 review citation

Applications of hydrogen deuterium exchange (HDX) for the characterization of conformational dynamics in light-activated photoreceptors.
Lindner et al. (2015)
6 other articles

1 mention without citation

Structure and function of a short LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae.
Endres et al. (2015)