› 4kuh

X-ray diffraction
2.51Å resolution

Crystal structure of 3-hydroxybutylryl-CoA dehydrogenase with acetoacetyl-CoA from Clostridium butyricum

Released:
DOI: 10.2210/pdb4kuh/pdb
PDB entry 4kuh coloured by chain, front view.
PDB entry 4kuh coloured by chain, side view.
PDB entry 4kuh coloured by chain, top view.
Source organism: Clostridium butyricum E4 str. BoNT E BL5262
Entry authors: Kim EJ, Kim S, Kim KJ

Function and Biology Details

Reaction catalysed: 
(S)-3-hydroxybutanoyl-CoA + NADP(+) = 3-acetoacetyl-CoA + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxybutyryl-CoA dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 286 amino acids
Theoretical weight: 31.11 KDa
Source organism: Clostridium butyricum E4 str. BoNT E BL5262
Expression system: Escherichia coli
UniProt:
  • Canonical: C4IEM5 (Residues: 1-280; Coverage: 99%)
Gene names: CLP_3850, hbd
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CAA 5 x CAA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: R3
Unit cell:
a: 146.338Å b: 146.338Å c: 409.757Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.191 0.257
Expression system: Escherichia coli

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