4kny

X-ray diffraction
2.94Å resolution

Crystal structure of the response regulator KdpE complexed to DNA in an active-like conformation

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
KDP operon transcriptional regulatory protein KdpE Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 25.5 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P21866 (Residues: 3-225; Coverage: 99%)
Gene names: JW5096, b0694, kdpE
Structure domains:
Promoter DNA Chain: Y
Molecule details ›
Chain: Y
Length: 30 nucleotides
Theoretical weight: 9.03 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
Promoter DNA Chain: Z
Molecule details ›
Chain: Z
Length: 30 nucleotides
Theoretical weight: 9.42 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P43212
Unit cell:
a: 133.14Å b: 133.14Å c: 133.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.209 0.248
Expression systems:
  • Escherichia coli
  • Not provided