4knw

X-ray diffraction
2.7Å resolution

The crystal structure of human HDHD4 IN COMPLEX WITH MAGNESIUM AND THE PHOSPHATE MIMETIC VANADATE

Released:

Function and Biology Details

Reaction catalysed:
N-acylneuraminate 9-phosphate + H(2)O = N-acylneuraminate + phosphate
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acylneuraminate-9-phosphatase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 257 amino acids
Theoretical weight: 28.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TBE9 (Residues: 2-248; Coverage: 100%)
Gene names: C20orf147, HDHD4, NANP
Sequence domains: Haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 46.777Å b: 102.56Å c: 186.665Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.254 0.296
Expression system: Escherichia coli BL21(DE3)