› 4kk6

X-ray diffraction
3.18Å resolution

Structure of CLC-ec1 deltaNC construct in 20mM Bromide

Released:
DOI: 10.2210/pdb4kk6/pdb
PDB entry 4kk6 coloured by chain, front view.
PDB entry 4kk6 coloured by chain, side view.
PDB entry 4kk6 coloured by chain, top view.
Source organisms:
Primary publication:
Fluoride-dependent interruption of the transport cycle of a CLC Cl-/H+ antiporter.
Lim HH, Stockbridge RB, Miller C
Nat Chem Biol 9 721-5 (2013)
PMID: 24036509

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
hetero dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-153303 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 446 amino acids
Theoretical weight: 47.62 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37019 (Residues: 17-460; Coverage: 94%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
Fab, heavy chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab, light chain Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand BR 4 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 231.362Å b: 100.918Å c: 171.718Å
α: 90° β: 132.12° γ: 90°
R-values:
R R work R free
0.237 0.235 0.269
Expression systems:
  • Escherichia coli
  • Mus musculus

Quick links

Citations

3 review citations

DENTAL ENAMEL FORMATION AND IMPLICATIONS FOR ORAL HEALTH AND DISEASE.
Lacruz et al. (2017)
2 more

1 mention without citation

Single-Molecule FRET of Membrane Transport Proteins.
Bartels et al. (2021)