› 4kjp

X-ray diffraction
3.2Å resolution

Structure of the CLC-ec1 deltaNC construct in the absence of halide

Released:
DOI: 10.2210/pdb4kjp/pdb
PDB entry 4kjp coloured by chain, front view.
PDB entry 4kjp coloured by chain, side view.
PDB entry 4kjp coloured by chain, top view.
Source organisms:
Primary publication:
Fluoride-dependent interruption of the transport cycle of a CLC Cl-/H+ antiporter.
Lim HH, Stockbridge RB, Miller C
Nat Chem Biol 9 721-5 (2013)
PMID: 24036509

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-219734 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 446 amino acids
Theoretical weight: 47.62 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37019 (Residues: 16-460; Coverage: 94%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
heavy chain of Fab fragment Chains: C, E
Molecule details ›
Chains: C, E
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
light chain of Fab fragment Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 232.449Å b: 98.246Å c: 171.026Å
α: 90° β: 132.08° γ: 90°
R-values:
R R work R free
0.224 0.222 0.265
Expression systems:
  • Escherichia coli
  • Mus musculus

Quick links

Citations

3 review citations

DENTAL ENAMEL FORMATION AND IMPLICATIONS FOR ORAL HEALTH AND DISEASE.
Lacruz et al. (2017)
2 more

2 mentions without citation

Localization atomic force microscopy.
Heath et al. (2021)
1 more