4kis

X-ray diffraction
3.2Å resolution

Crystal Structure of a LSR-DNA Complex

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Putative integrase [Bacteriophage A118] Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 328 amino acids
Theoretical weight: 39.16 KDa
Source organism: Listeria innocua Clip11262
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q928V6 (Residues: 133-452; Coverage: 71%)
Gene name: int
Sequence domains:
Structure domains: Arc Repressor Mutant, subunit A
DNA (26-MER) Chains: E, G, I, K
Molecule details ›
Chains: E, G, I, K
Length: 26 nucleotides
Theoretical weight: 7.91 KDa
Source organism: Listeria innocua Clip11262
Expression system: Not provided
DNA (26-MER) Chains: F, H, J, L
Molecule details ›
Chains: F, H, J, L
Length: 26 nucleotides
Theoretical weight: 8.07 KDa
Source organism: Listeria innocua Clip11262
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I23
Unit cell:
a: 290.75Å b: 290.75Å c: 290.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.236 0.256
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided