4kev

X-ray diffraction
2.65Å resolution

Crystal structure of SsoPox W263L

Released:
Source organism: Saccharolobus solfataricus
Entry authors: Gotthard G, Hiblot J, Chabriere E, Elias M

Function and Biology Details

Reaction catalysed:
An aryl dialkyl phosphate + H(2)O = dialkyl phosphate + an aryl alcohol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aryldialkylphosphatase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 314 amino acids
Theoretical weight: 35.58 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97VT7 (Residues: 1-314; Coverage: 100%)
Gene names: SSO2522, php
Sequence domains: Phosphotriesterase family
Structure domains: Metal-dependent hydrolases

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 83.906Å b: 105.075Å c: 153.888Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.25
Expression system: Escherichia coli BL21(DE3)