› 4kaw

X-ray diffraction
2.5Å resolution

Crystal structure of ribosome recycling factor mutant R39G from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb4kaw/pdb
PDB entry 4kaw coloured by chain, front view.
PDB entry 4kaw coloured by chain, side view.
PDB entry 4kaw coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Molecular flexibility of Mycobacterium tuberculosis ribosome recycling factor and its functional consequences: an exploration involving mutants.
Selvaraj M, Govindan A, Seshadri A, Dubey B, Varshney U, Vijayan M
J Biosci 38 845-55 (2013)
PMID: 24296887

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome-recycling factor Chain: X
Molecule details ›
Chain: X
Length: 185 amino acids
Theoretical weight: 20.76 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGY1 (Residues: 1-185; Coverage: 100%)
Gene names: MTCY274.13c, Rv2882c, frr
Sequence domains: Ribosome recycling factor
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CD 1 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 56.36Å b: 33.59Å c: 60.89Å
α: 90° β: 112.84° γ: 90°
R-values:
R R work R free
0.236 0.235 0.274
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Comparative secretomics reveals novel virulence-associated factors of Vibrio parahaemolyticus.
He et al. (2015)

1 mention without citation

A Structural View at Vaccine Development against M. tuberculosis.
Romano et al. (2023)