X-ray diffraction
2.24Å resolution

Crystal Structure of the His281Tyr/Ala460Ile Double Mutant of Benzoylformate Decarboxylase from Pseudomonas putida

Source organism: Pseudomonas putida
Entry authors: Brodkin HR, McLeish MJ

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Benzoylformate decarboxylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 533 amino acids
Theoretical weight: 57.17 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P20906 (Residues: 2-528; Coverage: 100%)
Gene name: mdlC
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand TPP 4 x TPP
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 70.769Å b: 164.546Å c: 175.881Å
α: 90° β: 90° γ: 90°
R R work R free
0.174 0.174 0.201
Expression system: Escherichia coli BL21(DE3)