4k8r

X-ray diffraction
3.22Å resolution

An Antibody Against the C-terminal Domain of PCSK9 lowers LDL Cholesterol Levels in vivo

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Proprotein convertase subtilisin/kexin type 9 Chain: A
Molecule details ›
Chain: A
Length: 92 amino acids
Theoretical weight: 10.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NBP7 (Residues: 61-152; Coverage: 14%)
Gene names: NARC1, PCSK9, PSEC0052
Sequence domains: Peptidase inhibitor I9
Structure domains: Peptidase S8 propeptide/proteinase inhibitor I9
Proprotein convertase subtilisin/kexin type 9 Chain: B
Molecule details ›
Chain: B
Length: 548 amino acids
Theoretical weight: 58.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NBP7 (Residues: 153-692; Coverage: 82%)
Gene names: NARC1, PCSK9, PSEC0052
Sequence domains:
Structure domains:
Fab1, light chain Chain: C
Molecule details ›
Chain: C
Length: 214 amino acids
Theoretical weight: 23.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab1, heavy chain Chain: D
Molecule details ›
Chain: D
Length: 234 amino acids
Theoretical weight: 24.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab3H42, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 238 amino acids
Theoretical weight: 25.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab3H42, light chain Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 22.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 262.19Å b: 138.65Å c: 69.54Å
α: 90° β: 102.87° γ: 90°
R-values:
R R work R free
0.17 0.167 0.226
Expression system: Escherichia coli