4k2p

X-ray diffraction
1.98Å resolution

The Structure of a Quintuple Mutant of the Tiam1 PH-CC-Ex Domain

Released:
Source organism: Homo sapiens
Primary publication:
High-resolution structure of the Tiam1 PHn-CC-Ex domain.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 69 744-52 (2013)
PMID: 23832200

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho guanine nucleotide exchange factor TIAM1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 276 amino acids
Theoretical weight: 31.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli M15
UniProt:
  • Canonical: Q13009 (Residues: 429-702; Coverage: 17%)
Gene name: TIAM1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21
Unit cell:
a: 80.507Å b: 70.111Å c: 87.753Å
α: 90° β: 90.19° γ: 90°
R-values:
R R work R free
0.194 0.192 0.236
Expression system: Escherichia coli M15