› 4jtd

X-ray diffraction
2.54Å resolution

Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Lys27Met mutant of Charybdotoxin

Released:
DOI: 10.2210/pdb4jtd/pdb
PDB 4jtd coloured by chain and viewed from the front.
PDB 4jtd coloured by chain and viewed from the side.
PDB 4jtd coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Structure of a pore-blocking toxin in complex with a eukaryotic voltage-dependent K(+) channel.
Banerjee A, Lee A, Campbell E, Mackinnon R
OpenAccess logo Elife 2 e00594 (2013)
PMID: 23705070

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero octamer
hetero nonamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Voltage-gated potassium channel subunit beta-2 Chains: A, G
Molecule details ›
Chains: A, G
Length: 333 amino acids
Theoretical weight: 37.35 KDa
Source organism: Rattus norvegicus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P62483 (Residues: 36-367; Coverage: 91%)
Gene names: Ckbeta2, Kcnab2, Kcnb3
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain
Potassium voltage-gated channel subfamily A member 2; Potassium voltage-gated channel subfamily B member 1 Chains: B, H
Molecule details ›
Chains: B, H
Length: 514 amino acids
Theoretical weight: 58.82 KDa
Source organism: Rattus norvegicus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P15387 (Residues: 30-30, 274-307; Coverage: 4%)
  • Canonical: P63142 (Residues: 1-31, 33-266, 305-499; Coverage: 92%)
Gene names: Kcna2, Kcnb1
Sequence domains:
Structure domains:
Potassium channel toxin alpha-KTx 1.1 Chain: Y
Molecule details ›
Chain: Y
Length: 37 amino acids
Theoretical weight: 4.31 KDa
Source organism: Leiurus quinquestriatus hebraeus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13487 (Residues: 23-59; Coverage: 100%)
Sequence domains: Scorpion short toxin, BmKK2

Ligands and Environments

3 bound ligands:
Ligand NAP 2 x NAP
Ligand K 8 x K
Ligand PGW 17 x PGW
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P4212
Unit cell:
a: 144.867Å b: 144.867Å c: 284.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.235
Expression systems:
  • Komagataella pastoris
  • Escherichia coli

Quick links

Citations

21 review citations

Animal toxins - Nature's evolutionary-refined toolkit for basic research and drug discovery.
Herzig et al. (2020)
20 more

1 mention without citation

Structure of a pore-blocking toxin in complex with a eukaryotic voltage-dependent K(+) channel.
Banerjee et al. (2013)