4jnx

X-ray diffraction
1.95Å resolution

Crystal structure of RNA silencing suppressor p19 complexed with double-helical RNA 20mer pG(CUG)6C

Released:
Source organism: Tomato bushy stunt virus
Entry authors: Katorcha E, Popov AN, Malinina L

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA silencing suppressor p19 Chains: A, D
Molecule details ›
Chains: A, D
Length: 127 amino acids
Theoretical weight: 14.51 KDa
Source organism: Tomato bushy stunt virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P69517 (Residues: 27-149; Coverage: 72%)
Gene name: ORF4
Structure domains: RNA silencing suppressor P19
5'-R(P*GP*CP*UP*GP*CP*UP*GP*CP*UP*GP*CP*UP*GP*CP*UP*GP*CP*UP*GP*C)-3' Chains: B, G
Molecule details ›
Chains: B, G
Length: 20 nucleotides
Theoretical weight: 6.34 KDa
Source organism: Tomato bushy stunt virus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: R3
Unit cell:
a: 89.917Å b: 89.917Å c: 148.353Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.234
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided